![]() Using a mathematical approach, a reasonable estimate was made to calculate the volume of plasma in which the dye was first diluted. The investigators verified that the concentration of the dye after mixing was not constant because it “disappeared” from blood plasma. In 1915, the dilution principle was first used in the study of human body composition when the use of a red dye to measure the plasma volume was extrapolated. Water's characteristic as a singular molecular species offers itself to the use of the dilution principle, which in its simplest form, states that the volume of the component is equal to the amount of isotope added to the component divided by the concentration of the isotope in that component. Unlike the other molecular body components, the water component consists of a single molecular species (H 2O), which simplifies the task of its measurement. Because it is mostly found in the fat-free body in a relatively constant amount, assessment of body water has been of interest as a method of body composition assessment for almost 100 years. Water is the most abundant component in the human body comprising about 60% of body mass in the reference man. This is an excerpt from ACSM's Body Composition Assessment With Web Resource by American College of Sports Medicine,Timothy Lohman & Laurie Milliken.īy Robert M.
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